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Image Search Results
Journal: Cell and tissue research
Article Title: Transcriptomic and Ultrastructural Evidence Indicate That Anti-HMGB1 Antibodies Rescue Organic Dust Induced Mitochondrial Dysfunction
doi: 10.1007/s00441-022-03602-3
Figure Lengend Snippet: Three experimental designs were used to study the impact of HMGB1 on mitochondria during ODE exposure. For acute exposure, primary NHBE cells were transfected with HMGB1 targeted siRNA to induce knockdown followed by exposure to either media (control) or ODE (1%) for 24 h (a). For chronic exposure, NHBE cells were treated with media alone (control) or ODE (1%) followed by media (control) or HMGB1 neutralization antibody (10 μg/mL) for 8 h per day for five days (b). Air-liquid interface (ALI) culture model was developed by seeing NHBE cells onto a semi-permeable membrane to develop and the differentiated cells were exposed to ODE, with or without HMGB1 neutralization for 1 h per day for 5 days (c).
Article Snippet: We purchased
Techniques: Transfection, Knockdown, Control, Neutralization, Membrane
Journal: Cell and tissue research
Article Title: Transcriptomic and Ultrastructural Evidence Indicate That Anti-HMGB1 Antibodies Rescue Organic Dust Induced Mitochondrial Dysfunction
doi: 10.1007/s00441-022-03602-3
Figure Lengend Snippet: NHBE cells differentiated on air-liquid interface (ALI) culture treated with medium or ODE (1%) followed by medium or HMGB1 neutralization antibody (10 μg/mL) for 1 h per day for 5 days. mRNA fold change of markers of motile cilia, foxj1 (a) and cfap157 (b), and tight junction, ocln (c) and cldn 1 (d), was measured by qPCR. Tight junction integrity was assessed by trans-epithelial electrical resistance (TEER) measurement (e). Samples for all assays were derived from the same experiment and were processed in parallel. Data was analyzed using one-way ANOVA with Tukey’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and represented as mean ± SEM with n = 3/treatment (* indicates significant difference from control).
Article Snippet: We purchased
Techniques: Neutralization, Derivative Assay, Comparison, Control